Estación Biológica Cocha Cashu

Association of the Endobiont Double-Stranded RNA Virus LRV1 With Treatment Failure for Human Leishmaniasis Caused by Leishmania braziliensis in Peru and Bolivia

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Revista Científica:

The Journal of Infectious Diseases

Año:

2015

Volumen:

213

Número:

1

Páginas:

112–121

DOI:

https://doi.org/10.1093/infdis/jiv354

ISSN:

0022-1899 (impreso), 1537-6613 (electrónico)

Keywords (Autor):

leishmaniasis, L. braziliensis, antimony drug treatment, RNA viruses, Viannia, drug treatment failure, drug resistance, Totivirus

Resumen

Cutaneous and mucosal leishmaniasis, caused in South America by Leishmania braziliensis, is difficult to cure by chemotherapy (primarily pentavalent antimonials [SbV]). Treatment failure does not correlate well with resistance in vitro, and the factors responsible for treatment failure in patients are not well understood. Many isolates of L. braziliensis (>25%) contain a double-stranded RNA virus named Leishmaniavirus 1 (LRV1), which has also been reported in Leishmania guyanensis, for which an association with increased pathology, metastasis, and parasite replication was found in murine models. Here we probed the relationship of LRV1 to drug treatment success and disease in 97 L. braziliensis–infected patients from Peru and Bolivia. In vitro cultures were established, parasites were typed as L. braziliensis, and the presence of LRV1 was determined by reverse transcription–polymerase chain reaction, followed by sequence analysis. LRV1 was associated significantly with an increased risk of treatment failure (odds ratio, 3.99; P = .04). There was no significant association with intrinsic SbV resistance among parasites, suggesting that treatment failure arises from LRV1-mediated effects on host metabolism and/or parasite survival. The association of LRV1 with clinical drug treatment failure could serve to guide more-effective treatment of tegumentary disease caused by L. braziliensis.

Copyright:

© 2015 The Author.

Autor (es ):

Adaui,Vanessa; Lye, Lon-Fye; Akopyants, Natalia S.; Zimic, Mirko; Llanos-Cuentas,Alejandro; Garcia, Lineth; Maes, Ilse; De Doncker, Simonne; Dobson,Deborah E.; Arevalo, Jorge; Dujardin, Jean-Claude; Beverley, Stephen M.

Editorial:

Oxford University Press

Ciudad:

Oxford, Reino Unido

Idioma:

Inglés

Tipo de Referencia:

Articulo de revista academica

Investigadores: